医药学论文：某些心血管疾病的发生与血液动力学改变的关系[1]

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关键字：水平 病毒 血浆 观察 药物 模型 艾滋病 感染 滴度 病毒血症　　摘要：目的：在先前已建立了模拟人艾滋病感染者和患者的猴免疫缺陷病毒(SIV)急性感染猴和中、晚期猴艾滋病(SAIDS)模型的基础上，再摸索建立SIV慢性感染猴模型，以期为抗艾滋病药物的体内药效学评价研究提供更合适的动物模型。方法：用SIVmac251感染恒河猴17只，追踪观察其临床表现，体征变化，病程进展，血浆和全血病毒血症规律，淋巴细胞亚群CD4的动态等。结果：临床体征有特征性，病程较长，1年内死亡2只(11.8%)。血浆病毒血症水平在感染后14d达高峰，感染21d开始下降，60～360d检不出或低滴度。全血病毒血症水平在感染后10d开始持续高滴度至3个月，3个月后仍有半数以上持续高滴度，9个月后才下降。淋巴细胞亚群CD4值感染后14～30d略下降，后回升。结论：SIV慢性感染猴模型临床表现、病毒学和免疫学的变化及病程进展类似人艾滋病感染者在进入艾滋病中、晚期前的慢性感染过程，可用于抗艾滋病药物疗效的研究。

　

Establishment of Monkey Model with Chronic Infection of

　　Simian Acquired Immunodeficiency Virus

　　Abstract: Further exploration of the establishment of monkey model chronically infected with Simian acquired immunodeficiency virus (SIV) was conducted on the basis of previous studies of SIV monkey models and simian acquired immunodeficiency syndrome models. Seventeen Rhesus monkeys were infected with SIVmac?251. Items such as symptoms and signs, course of infection,plasma viremia and the whole blood, changes of CD4 lymphocyte subg roup were observed periodically. The results showed that specific clinical manifestations occurred, disease course prolonged and 2 of 17 monkeys died of severe immunodeficiency. Plasma viremia reached the peak on 14th day after infection, began to decrease on 21st day, and remained low titer or underdetermined from 60th to 360th day. Whole blood viremia reached high titer from 10th to 90th day, and over half of the monkeys maintained high viremia level up to 9th month and then decreased afterwards. CD4 lymphocyte level decreased from 14th to 30th day, and then increased. The above changes of clinical manifestations, virology and immunology are similar to cases with chronic infection of human immunodeficiency virus (HIV) in the early stage. And this will provide a suitable animal model for the research of anti-AIDS drugs.

　　Key words: SIMIAN ACQUIRED IMMUNODEFICIENCY SYNDROMES; DISEASE MODELS, ANIMAL;　MACACA

　　猴免疫缺陷病毒(SIV)　感染猴和模型猴因其发病过程和发病机理酷似人免疫缺陷综合医药学论文