英语翻译At low magnification,single cells grew as a homogeneous monolayer of cells with cobblestone-like appearance.The cells were structurally polarized:the apical pole,facing the free surface,differed from the basal pole,toward the underlying s

英语翻译At low magnification,single cells grew as a homogeneous monolayer of cells with cobblestone-like appearance.The cells were structurally polarized:the apical pole,facing the free surface,differed from the basal pole,toward the underlying s
英语翻译
At low magnification,single cells grew as a homogeneous monolayer of cells with cobblestone-like appearance.The cells were structurally polarized:the apical pole,facing the free surface,differed from the basal pole,toward the underlying support layer.The presence of specific markers,like transporter proteins or brush border enzymes,was established to characterize these primary cultures.The PTC shows a clear immunoreactivity against AQP1,while no positive signal for early distal tubule expressing AQP2 or NCC was obtained.The multiligand receptor,megalin,is located at the brush border of PT cells and participates in protein reabsorption from the primitive urine.The chloride channel,ClC-5,and the vacuolar proton pump,V-ATPase,are both localized in endosomes ensuring correct endosomal acidification and protein trafficking in PT cells.All of these components of the endocytic apparatus were detected in primary PT cells

英语翻译At low magnification,single cells grew as a homogeneous monolayer of cells with cobblestone-like appearance.The cells were structurally polarized:the apical pole,facing the free surface,differed from the basal pole,toward the underlying s
低倍镜下,成长为一个单个细胞的细胞与cobblestone-like单层均匀.这些细胞是结构性的顶端极极化,面向自由面,有别于基底杆,向潜在支持层.在场的情况下,如转运蛋白的特殊标记或刷边境酶,建立了描述这些主要的文化.PTC显示immunoreactivity攻击,而不积极的信号AQP1表达AQP2远为早期发生.应或者这个multiligand受体,megalin,位于边境的笔刷和参与PT细胞蛋白质增高从原始的尿液.氯离子通道,ClC-5、金属质子泵、V-ATPase,都是确保正确定位endosomes内酸化和蛋白质贩卖人口的细胞.PT所有这些部件的endocytic仪进行检测的主要PT细胞

在低倍率，单细胞成长为一个单一的细胞单层鹅卵石般的外观。这些细胞在结构分化：顶柱，面对自由表面，不同於基底极，对潜在的支持层。存在的具体指标，如转运蛋白或刷状缘酶，成立这些主要的文化表徵。 PTC的显示了一个明确的免疫反应对水通道蛋白1，而没有任何积极的信号提前或远端小管表达 AQP2全国协商委员会获得。该 multiligand受体，这种蛋白质是位於细胞刷状缘的PT和参与蛋白质的重吸收，由原始的...

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在低倍率，单细胞成长为一个单一的细胞单层鹅卵石般的外观。这些细胞在结构分化：顶柱，面对自由表面，不同於基底极，对潜在的支持层。存在的具体指标，如转运蛋白或刷状缘酶，成立这些主要的文化表徵。 PTC的显示了一个明确的免疫反应对水通道蛋白1，而没有任何积极的信号提前或远端小管表达 AQP2全国协商委员会获得。该 multiligand受体，这种蛋白质是位於细胞刷状缘的PT和参与蛋白质的重吸收，由原始的尿液。氯离子通道，氯通道ClC - 5，和液泡质子泵，V - ATP酶，都定位於内涵体确保正确的胞内酸化及蛋白贩运星期三细胞。所有这些组成部分的内吞仪器检测细胞在小学星期三

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