tris maleate缓冲液怎么配

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tris maleate缓冲液怎么配
tris maleate缓冲液怎么配

tris maleate缓冲液怎么配
Tris-maleate Buffer pH 5.8-8.2
Stock solution:0.2M liter
Tris(hydroxymethyl)aminomethane 24.2 gm
Maleic acid 23.2 gm
HO2CCH:CHCO2H (MW = 116.07)
+ ddH20 to make 1 liter
或者
Trizima-maleate (MW = 237.2) 47.4 gm
+ ddH20 to make 1 liter
Working buffer:0.2M 100 ml
Adjust 50 ml of stock solution to desired pH with 0.1M NaOH.Dilute to 100 ml with ddH20.