英语翻译3.1.Growth of mixed cultures under carbon,nitrate and phosphate limitationFig.1 shows the mean concentrations of glucose,nitrate and phosphate measured in the carbon-,nitrate- and phosphate-limited mixed continuous cultures.In the four ca

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英语翻译3.1.Growth of mixed cultures under carbon,nitrate and phosphate limitationFig.1 shows the mean concentrations of glucose,nitrate and phosphate measured in the carbon-,nitrate- and phosphate-limited mixed continuous cultures.In the four ca
英语翻译
3.1.Growth of mixed cultures under carbon,nitrate and phosphate limitation
Fig.1 shows the mean concentrations of glucose,nitrate and phosphate measured in the carbon-,nitrate- and phosphate-limited mixed continuous cultures.In the four carbon-limited cultures,glucose (Fig.1A) was always below the limit of detection (15 μM).In the other cultures,residual glucose (supplied at 0.7 mM in the growth medium) was sometimes present.It should be noted that in all cultures,the growth medium also contained acetate,citrate,benzoate and salicylate as additional carbon and energy sources.Nitrate (Fig.1B) was detectable (0.5 mM) in the surface water used for inoculation,but in the four nitratelimited cultures,nitrate was no longer detectable (650μM) from the first sampling 68 h after switching on the medium supply pump.In the carbon- and phosphate-limited cultures the average nitrate concentration was lower than 2.5 mM during the whole period,indicating that significant bacterial consumption of nitrate occurred also in these cultures.Fig.1C shows the residual concentration of phosphate in the cultures.No phosphate could be detected under phosphate limitation.The amount of phosphate supplied in the medium (20 μM) was just above the limit of detection (10 μM).In the carbon- and nitrate-limited chemostats phosphate (10 mM) was evidently supplied to the cultures in excess.Since nitrate (Fig.1B) and occasionally glucose (Fig.1A) could be detected in the phosphatelimited cultures and since the molar ratio of phosphate per carbon supplied was only 0.0014:1,growth of the mixed bacterial culture on the medium with proportionally less phosphate was concluded to be controlled by the amount of phosphate.Fig.2 provides an additional indication that the cultures that received less phosphate were limited by this macronutrient.The specific cell-bound activity of APase was consistently about 10-fold lower in the other cultures that contained 10 mM of phosphate in the growth medium.

英语翻译3.1.Growth of mixed cultures under carbon,nitrate and phosphate limitationFig.1 shows the mean concentrations of glucose,nitrate and phosphate measured in the carbon-,nitrate- and phosphate-limited mixed continuous cultures.In the four ca
图 1 表演在碳被测量的葡萄糖,硝酸盐和磷酸盐的低劣集中-,硝酸盐- 而且磷酸盐-有限使连续的文化混合了.在四碳-有限耕种,葡萄糖 (图 1A) 总是在发现 (15 μ M) 的界限下面.在其他文化方面,剩余葡萄糖 (在生长媒体中的 0.7 毫莫耳供应) 有时在场.它应该被注意所有的文化的那,生长媒体也包含了如另外的碳和能源来源的醋酸盐,柠檬酸盐,安息香酸盐和水杨酸盐.硝酸盐 (图 1B) 在作为接插芽的地表水中是可发觉的 (0.5 毫莫耳),但是在四 nitratelimited 文化方面,硝酸盐从那首先在打开萣生熟的补给泵之后抽取样品 68 h 不再可发觉的 (650 μ M).在碳- 和磷酸盐-有限耕种集中在整个期间比 2.5 毫莫耳低,指出硝酸盐的重要细菌消费也在这些文化方面发生了的平均的硝酸盐.文化的图 1C 表演磷酸盐的剩余集中.没有磷酸盐可能在磷酸盐限制之下被发现.在媒体 (20 μ M) 被供应的磷酸盐的数量是确实上述发现 (10 μ M) 的界限.在碳- 和硝酸盐-有限的 chemostats 磷酸盐 (10 毫莫耳) 明显地被供应到过度的文化.自从硝酸盐 (图 1B) 以后和有时候葡萄糖 (图 1A) 可能在 phosphatelimited 文化方面被发现而且自从被供应的每碳磷酸盐的磨碎比之后是只有 0.0014:1,混合细菌的文化的生长在和比例比较少的磷酸盐的媒体上被总结被磷酸盐的数量控制.图 2 提供另外的指示收到了比较少的磷酸盐的文化被这 macronutrient 限制了.APase 的特定细胞-范围活动是一致地大约 10-折层在生长媒体中包含了 10 毫莫耳的磷酸盐的其他文化方面降低.

3.1. 在碳,硝酸盐和磷酸盐限制下面的混合文化的生长
图 1 表演在碳被测量的葡萄糖,硝酸盐和磷酸盐的低劣集中-, 硝酸盐- 而且磷酸盐-有限使连续的文化混合了。在四碳-有限耕种, 葡萄糖 (图 1A) 总是在发现 (15 μ M) 的界限下面. 在其他文化方面,剩余葡萄糖 (在生长媒体中的 0.7 毫莫耳供应) 有时在场。 它应该被注意所有的文化的那,生长媒体也包含了如另外的碳和能源来...

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3.1. 在碳,硝酸盐和磷酸盐限制下面的混合文化的生长
图 1 表演在碳被测量的葡萄糖,硝酸盐和磷酸盐的低劣集中-, 硝酸盐- 而且磷酸盐-有限使连续的文化混合了。在四碳-有限耕种, 葡萄糖 (图 1A) 总是在发现 (15 μ M) 的界限下面. 在其他文化方面,剩余葡萄糖 (在生长媒体中的 0.7 毫莫耳供应) 有时在场。 它应该被注意所有的文化的那,生长媒体也包含了如另外的碳和能源来源的醋酸盐,柠檬酸盐,安息香酸盐和水杨酸盐。 硝酸盐 (图 1B) 在作为接插芽的地表水中是可发觉的 (0.5 毫莫耳), 但是在四 nitratelimited 文化方面,硝酸盐从那首先在打开萣生熟的补给泵之后抽取样品 68 h 不再可发觉的 (650 μ M)。在碳- 和磷酸盐-有限耕种集中在整个期间比 2.5 毫莫耳低,指出硝酸盐的重要细菌消费也在这些文化方面发生了的平均的硝酸盐。 文化的图 1C 表演磷酸盐的剩余集中。 没有磷酸盐可能在磷酸盐限制之下被发现。 在媒体 (20 μ M) 被供应的磷酸盐的数量是确实上述发现 (10 μ M) 的界限。 在碳- 和硝酸盐-有限的 chemostats 磷酸盐 (10 毫莫耳) 明显地被供应到过度的文化。 自从硝酸盐 (图 1B) 以后和有时候葡萄糖 (图 1A) 可能在 phosphatelimited 文化方面被发现而且自从被供应的每碳磷酸盐的磨碎比之后是只有 0.0014:1,混合细菌的文化的生长在和比例比较少的磷酸盐的媒体上被总结被磷酸盐的数量控制。 图 2 提供另外的指示收到了比较少的磷酸盐的文化被这 macronutrient 限制了。APase 的特定细胞-范围活动是一致地大约 10-折层在生长媒体中包含了 10 毫莫耳的磷酸盐的其他文化方面降低。

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